In Vivo Scintigraphic Imaging of Antitumor Effects by Combined Radioiodine Therapy and Human Sodium Iodide Symporter Gene Immunotherapy

被引:0
|
作者
Choi, Yun
Jeon, Yong Hyun
Paik, Jin Ho
Ko, Jinkyung
Choi, Doe Han
Chung, June Key
Kim, Chul Woo [1 ]
机构
[1] Seoul Natl Univ, Coll Med, Dept Pathol, Tumor Immun Med Res Ctr, Seoul 110744, South Korea
来源
MOLECULAR IMAGING | 2010年 / 9卷 / 03期
关键词
REGULATORY T-CELLS; DNA VACCINE; PROSTATE-CANCER; IONIZING-RADIATION; THYROID-CANCER; ANTIGEN; EXPRESSION; CARCINOMA; RADIOTHERAPY; IRRADIATION;
D O I
10.2310/7290.2010.00010
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In a previous study, we demonstrated that pcDNA3.1/hNIS (human sodium iodide symporter) vaccination generated hNIS-associated CD8(+)IFN-gamma(+) (interferon-gamma) T cells, which are known to be involved in antitumor immunity. However, the immune response induced was insufficient to control tumor growth in vivo, which required a novel approach to potentiate hNIS vaccination effects. In the present study, we administered I-131 radioiodine therapy prior to hNIS vaccination in CT26/hNIS tumor-bearing mice to facilitate the vaccine-induced immune response. We characterized hNIS-associated cytotoxic T-cell immune response and the antitumor effects induced by this I-131 + hNIS combination therapy. The survival rates of CT26/hNIS tumor cells were significantly reduced by I-131 treatment compared with the parental CT26 cells in vitro. I-131 + hNIS combination therapy stably suppressed tumor growth below or near the original tumor size level of initial treatment, achieving 100% survival rates. Specifically, I-131 + hNIS therapy enhanced IFN-gamma production, hNIS-associated antitumor cytotoxic T-lymphocyte (CTL) response, and induced more dendritic cells but reduced T-regulatory cells in tumor masses. Collectively, these results suggest that combined therapy effectively enhances hNIS-associated antitumor immune response, leading to CT26/hNIS tumor growth inhibition and complete survival in Balb/C mice. These findings provide a novel and effective means of treating cancer.
引用
收藏
页码:141 / 152
页数:12
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