Generation and efficacy evaluation of a recombinant adenovirus expressing the E2 protein of classical swine fever virus

被引：37
|
作者：
Sun, Yuan [1 ]
Liu, Da-Fei [1 ]
Wang, Yu-Fei [1 ]
Liang, Bing-Bing [1 ]
Cheng, Dan [1 ]
Li, Na [1 ]
Qi, Qiao-Fen [1 ]
Zhu, Qing-Hu [1 ]
Qiu, Hua-Ji [1 ]
机构：
[1] Chinese Acad Agr Sci, Div Swine Infect Dis, Natl Key Lab Vet Biotechnol, Harbin Vet Res Inst, Harbin 150001, Peoples R China
来源：
RESEARCH IN VETERINARY SCIENCE | 2010年 / 88卷 / 01期
关键词：
Classical swine fever virus; E2; gene; Recombinant adenovirus; Efficacy; Rabbit; Pig; MOLECULAR CHARACTERIZATION; PORCINE ADENOVIRUS; PIGS; VACCINATION; PROTECTION; VACCINES; IMMUNIZATION; CHALLENGE; TRANSMISSION; GLYCOPROTEIN;
D O I：
10.1016/j.rvsc.2009.06.005
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), which causes significant economic losses to the pig industry worldwide. The E2 glycoprotein of CSFV is the main target for neutralizing antibodies. This study was aimed to develop a recombinant human adenovirus type 5 expressing the CSFV E2 gene (rAdV-E2) and evaluate its efficacy in rabbits and pigs. The results showed that the rabbits and the pigs immunized with the rAdV-E2 developed high-level CSFV-specific neutralizing antibodies. The rAdV-E2-immunized rabbits were protected from fever induced by infection with C-strain, which is pathogenic to the rabbit, and the rAdV-E2-immunized pigs were protected from lethal challenge with highly virulent Shimen strain. This indicates that the recombinant adenovirus can be an attractive candidate vaccine for preventing CSF. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码：77 / 82
页数：6
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