DISSIPATION OF DOUBLE-STRANDED RNA IN AQUATIC MICROCOSMS

被引：29

作者：

Albright, Vurtice C., III ^{[1
]}

Wong, Colin R. ^{[1
]}

Hellmich, Richard L. ^{[2
]}

Coats, Joel R. ^{[1
]}

机构：

[1] Iowa State Univ, Dept Entomol, Pesticide Toxicol Lab, Ames, IA USA

[2] ARS, Corn Insects & Crop Genet Res Unit, USDA, Ames, IA USA

来源：

ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY | 2017年 / 36卷 / 05期

关键词：

dsRNA; Double-stranded RNA; Environmental fate; Aquatic microcosms; BACILLUS-THURINGIENSIS; TARGETED ACTIVITY; DNA; INTERFERENCE; PERSISTENCE; FATE; GENE; SOIL; DEGRADATION; DSRNA;

D O I：

10.1002/etc.3648

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Silencing genes of a pest with double-stranded RNA (dsRNA) is a promising new pest management technology. As part of the environmental risk assessment for dsRNA-based products, the environmental fate and the potential for adverse effects to on-target organisms should be characterized. In the present study, a nonbioactive dsRNA was spiked into the water column of a water and sediment microcosm to mimic drift from a spray application run off of unbound dsRNA or transport of plant tissues. Dissipation of dsRNA in the water column and partitioning into sediment was determined. The dsRNA rapidly dissipated in the water column and was below the limit of detection after 96 h. The levels detected in the sediment were not significant and may indicate rapid degradation in the water column prior to partitioning to sediment. (C) 2016 SETAC

引用

页码：1249 / 1253

页数：5

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