Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB

被引：0
|
作者：
Zhang, TX [1 ]
Liu, HZ [1 ]
Chen, JY [1 ]
机构：
[1] Chinese Acad Sci, Lab Separat Sci & Engn, Inst Chem Met, Beijing 100080, Peoples R China
来源：
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY | 2000年 / 75卷 / 09期
关键词：
reversed micelles; alcohol dehydrogenase; protein purification; CTAB;
D O I：
10.1002/1097-4660(200009)75:9<798::AID-JCTB282>3.0.CO;2-P
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A large molecular weight protein, alcohol dehydrogenase (ADH, 141 kDa), has been purified fi om baker's yeast using reversed micelles formed with a cationic surfactant (cetyltrimethylammonium bromide, CTAB) using the phase transfer method. Various parameters, such as pH, ionic strength and contact time of the two phases in ADH forward and backward transfer were studied. The active ADH was successfully recovered after a full forward and backward extraction cycle. The recovery of ADH activity obtained was similar to 90% and the purification factor was 3.1 for the overall process. (C) 2000 Society of Chemical Industry.
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页码：798 / 802
页数：5
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