Separation of xylo-oligosaccharides from enzymatic hydrolytes using membrane reactor

The time course of xylo-oligosaccharides concentration and xylo-oligosaccharides yield in the separation xylo-oligosaccharides from enzymatic hydrolytes was studied using a membrane reactor with constant permeate flux of 4 L . m(-2) . h(-1). The results show that xylanases retain 90%, of its activity in the reactor. The concentration xylo-oligosaccharides achieves the maximum, about 5. 48 g/L in. 30 min. The difference of xylo-oligosaccharides in the retentate and permeate stream is low, <0. 62 g/L, therefore it can permeate through membrane. Under the operating conditions that xylan concentration is 30. 0 g/L, pH 5.0, operating pressure 16 kPa, temperature 48 degreesC, feed velocity 400 mL/min, reaction volume 400 mL, enzyme dosage 10%(volume fraction), dilution rate 1-h(-1), and enzymatic hydrolysis time 195 min, the yield of xylo-oligosaccharides reaches 31. 69%.