Direct detection of Streptococcus pneumoniae in positive blood cultures by real-time polymerase chain reaction

被引：9
|
作者：
Selva, Laura [1 ]
Esteva, Cristina [1 ]
Gene, Amadeu [1 ]
Fernandez de Sevilla, Maria [2 ]
Hernandez-Bou, Susanna [2 ]
Munoz-Almagro, Carmen [1 ]
机构：
[1] Univ Hosp St Joan de Deu, Dept Mol Microbiol, Esplugues Barcelona 08950, Spain
[2] Univ Hosp St Joan de Deu, Dept Paediat, Esplugues Barcelona 08950, Spain
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2010年 / 66卷 / 02期
关键词：
Real-time PCR; Blood culture bottles; Streptococcus pneumoniae; COMMUNITY-ACQUIRED PNEUMONIA; RAPID DETECTION; PCR; BOTTLES; IDENTIFICATION; CHILDREN; DNA;
D O I：
10.1016/j.diagmicrobio.2009.05.023
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
We developed a real-time polymerase chain reaction specific for Streptococcus pneumoniae to be applied directly from blood culture bottles without previous DNA extraction step. For the 128 blood culture bottles tested, the assay had 94% and 98.4% sensitivity and specificity, respectively. This assay provides rapid and accurate identification of this pathogen. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码：204 / 206
页数：3
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