UV photolysis of catalase revisited:: a spectral study of photolytic intermediates

被引:12
|
作者
Aubailly, M [1 ]
Haigle, J [1 ]
Giordani, A [1 ]
Morlière, P [1 ]
Santus, R [1 ]
机构
[1] Museum Natl Hist Nat, INSERM U532, Lab Photobiol, F-75231 Paris 05, France
关键词
catalase; photochemistry; compound II; compound III; ferrous catalase;
D O I
10.1016/S1011-1344(00)00061-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The 365-nm irradiation of 4.6 mu M (similar or equal to 1.1 mg/ml) catalase solutions in pH 7.4 phosphate buffer induces spectral modifications. Difference spectra show maxima at 434, 555, 584 nm at the beginning of the irradiation, then a final spectrum with a maximum at 568 nm and a shoulder at 530 nm is observed. These results suggest the formation of compound III (oxyferrous catalase) and compound II, respectively. In deaerated 0.1 M, pH 8.7 berate buffer, the ferrous catalase is characterized by maxima at 563 and 594 nm. Hydrogen donors such as ethyl alcohol, formate and p-cresol inhibit, but citrate ions enhance the formation of these intermediates. A mechanism involving Fe(III) reduction according to an internal electron transfer is proposed. (C) 2000 Elsevier Science S.A. All rights reserved.
引用
收藏
页码:61 / 67
页数:7
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