Characterization of shiga toxin-producing Escherichia coli strains isolated from calves in Poland

被引：38
|
作者：
Osek, J
Gallien, P
Protz, D
机构：
[1] PIWet, Natl Inst Vet Res, PL-24100 Pulawy, Poland
[2] BgVV, Fed Inst Hlth Protect Consumers & Vet Med, D-06846 Dessau, Germany
来源：
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2000年 / 23卷 / 04期
关键词：
E; coli; calves; diarrhea; shiga toxin; pathogenicity factors; PCR;
D O I：
10.1016/S0147-9571(00)00008-4
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Fecal samples from 67 3-5-months-old calves with diarrhea were screened for the presence of shiga toxin-producing Escherichia coli (STEC). Several accessory virulence factors genes were also tested. Among 192 E.coli isolates tested, 15 (7.6%) were found to harbour the shiga toxin 1 or 2 (stx1 or stx2) genes. The stx2-carrying samples were further subtyped by PCR for the stx2c, stx2d, and stx2e toxin variants. It was shown that stx2-positive bacteria mainly possessed the stx2e shiga toxin type gene. The enterohemolysin (hlyA) and intimin (ene) genes were found in seven (46.7%) STEC strains whereas the cytotoxic necrotizin factor 1 and 2 or the P fimbrial genes were detected in two isolates only. This study confirmed that calves are a reservoir of STEC strains (with all pathogenicity genes) that may be virulent for humans. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：267 / 276
页数：10
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