High level secretion of recombinant staphylokinase into periplasm of Escherichia coli

被引：20
|
作者：
Lee, SJ
Kim, IC
Kim, DM
Bae, KH
Byun, SM
机构：
[1] Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea
[2] Res Ctr New Biomat Agr, Taejon 305701, South Korea
来源：
BIOTECHNOLOGY LETTERS | 1998年 / 20卷 / 02期
关键词：
D O I：
10.1023/A:1005359920522
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The staphylokinase (SAK) gene from Staphylococcus aureus NCTC10033 was inserted into an expression vector, pKK-ompA, having a tac promoter and an ompA signal sequence. Escherichia coli JM109 carrying the recombinant plasmid produced and secreted the recombinant SAK (rSAK) at 15ug/ml into periplasm and 5ug/ml to extracellular media, respectively. The rSAK was purified with 59% yield by simple procedures from the periplasm of E. coli. The aminoterminal sequence and human plasminogen activating activity of rSAK were coincided with the authentic SAK.
引用
收藏
页码：113 / 116
页数：4
相关论文
共 50 条
  • [21] Folding LacZ in the Periplasm of Escherichia coli
    Dwyer, Robert S.
    Malinverni, Juliana C.
    Boyd, Dana
    Beckwith, Jon
    Silhavy, Thomas J.
    JOURNAL OF BACTERIOLOGY, 2014, 196 (18) : 3343 - 3350
  • [22] In vivo monitoring and alleviation of extracytoplasmic stress to recombinant protein overproduction in the periplasm of Escherichia coli
    Narayanan, Niju
    Follonier, Stephanie
    Chou, C. Perry
    BIOCHEMICAL ENGINEERING JOURNAL, 2008, 42 (01) : 13 - 19
  • [23] High-level accumulation of a recombinant antibody fragment in the periplasm of Escherichia coli requires a triple-mutant (degP prc spr) host strain
    Chen, C
    Snedecor, B
    Nishihara, JC
    Joly, JC
    McFarland, N
    Andersen, DC
    Battersby, JE
    Champion, KM
    BIOTECHNOLOGY AND BIOENGINEERING, 2004, 85 (05) : 463 - 474
  • [24] Production of bioactive recombinant monoclonal antibody fragment in periplasm of Escherichia coli expression system
    Saroha, Preeti
    Rathore, Anurag S.
    PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2023, 53 (10): : 1288 - 1296
  • [25] Intein-Mediated Cyclization of Randomized Peptides in the Periplasm of Escherichia coli and Their Extracellular Secretion
    Deschuyteneer, Genevieve
    Garcia, Stephanie
    Michiels, Benjamin
    Baudoux, Bruno
    Degand, Herve
    Morsomme, Pierre
    Soumillion, Patrice
    ACS CHEMICAL BIOLOGY, 2010, 5 (07) : 691 - 700
  • [26] HIGH-LEVEL SECRETION OF HUMAN CARDIODILATIN BY ESCHERICHIA-COLI
    SHIBUI, T
    UCHIDA, M
    NAGAHARI, K
    TERANISHI, Y
    AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1988, 52 (05): : 1145 - 1150
  • [27] High level secretion of a humanized bispecific diabody from Escherichia coli
    Zhu, ZP
    Zapata, G
    Shalaby, R
    Snedecor, B
    Chen, H
    Carter, P
    BIO-TECHNOLOGY, 1996, 14 (02): : 192 - 196
  • [28] HIGH-LEVEL EXPRESSION AND SECRETION OF STREPTOKINASE IN ESCHERICHIA-COLI
    KO, JH
    PARK, DK
    KIM, IC
    LEE, SH
    BYUN, SM
    BIOTECHNOLOGY LETTERS, 1995, 17 (10) : 1019 - 1024
  • [29] Removal of Residual DNA and Host Cell Proteins for the Purification of Recombinant Staphylokinase Expressed in Escherichia coli
    Li, Guang
    Li, Jing
    Zou, Zhenxing
    Liu, Bo
    Li, Weiping
    Ding, Yang
    Song, Xuri
    Fang, Xiaolan
    Hu, Daoqi
    SEPARATION SCIENCE PLUS, 2024, 7 (09):
  • [30] Single-step purification of recombinant anthrax lethal factor from periplasm of Escherichia coli
    Chang, Hsin-Hou
    Tsai, Mei-Fang
    Chung, Chia-Pei
    Chen, Po-Kong
    Hu, Hsin-I
    Kau, Jyh-Hwa
    Huang, Hsin-Hsien
    Lin, Hung-Chi
    Sun, Der-Shan
    JOURNAL OF BIOTECHNOLOGY, 2006, 126 (03) : 277 - 285
12345