A Loop-Mediated Isothermal Amplification Assay Targeting Neisseria gonorrhoeae penA-60.001

被引：6
|
作者：
Shimuta, Ken [1 ,2 ]
Nakayama, Shu-ichi [1 ]
Takahashi, Hideyuki [1 ]
Ohnishi, Makoto [1 ]
机构：
[1] Natl Inst Infect Dis, Dept Bacteriol 1, Tokyo, Japan
[2] Natl Inst Infect Dis, Antimicrobial Resistance Res Ctr, Tokyo, Japan
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2020年 / 64卷 / 01期
基金：
日本学术振兴会;
关键词：
Neisseria gonorrhoeae; ceftriaxone; loop-mediated isothermal amplification; LAMP; penA; PENA GENE; STRAIN; CEFTRIAXONE; RESISTANCE; FC428; LAMP;
D O I：
10.1128/AAC.01663-19
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Ceftriaxone (CRO) is widely used as the first-line treatment for gonococcal infections. However, CRO-resistant Neisseria gonorrhoeae strains carrying mosaic penA-60.001 have emerged recently and disseminated worldwide. To meet the urgent need to detect these strains, we report here a loop-mediated isothermal amplification (LAMP) assay system that targets N. gonorrhoeae penA-60.001. This assay system can differentiate N. gonorrhoeae strains carrying mosaic penA-60.001 from strains carrying other penA alleles.
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页数：5
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