Production of poly-N-acetylglucosamine by Staphylococcus saprophyticus BMSZ711: Characterization and production optimization

被引：2
|
作者：
Zamil, Sheikh Shawkat
Ahmad, Shabir
Choi, Mun Hwan
Yoon, Sung Chul [1 ]
机构：
[1] Gyeongsang Natl Univ, Grad Sch, Div Appl Life Sci BK21, Nanobiomat Sci Lab, Jinju 660701, South Korea
来源：
BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 18期
关键词：
Staphylococcus saprophyticus; Poly-N-acetylglucosamine (PNAG); Glucosamine; Exopolysaccharide; PNAG production optimization; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; D-GLUCOSAMINE; EXOPOLYSACCHARIDE; POLYSACCHARIDE; CHITIN; AUREUS; ACID;
D O I：
10.1016/j.biortech.2010.03.141
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
This is the first report on the characterization and production optimization of poly-N-acetylglucosamine (PNAG) in Staphylococcus saprophyticus A strain producing glucosamine exopolysaccharide was isolated and characterized by biochemical test and 16S rRNA gene sequence homology analysis and named as S saprophyticus BMSZ711 The molecular mass of the purified exopolymer was about 12 kDa. Digestion of the PNAG with Dispersin (R) proved that it has beta-1.6 linkage BMSZ711 can only produce PNAG when grown in M1 minimal medium but not in nutrient rich medium with optimum temperature of 30 degrees C and pH of 7 Glycerol and ammonium sulfate were found to be the best carbon and nitrogen source, respectively Maximum PNAG production was obtained when glycerol 100 mM, ammonium sulfate 0.3%, yeast extract 1.5 g/L, sodium chloride 10 g/L and valine 2 mM were used. (C) 2010 Elsevier Ltd All rights reserved
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页码：7177 / 7180
页数：4
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