Optimization studies on the production of cyclosporin A by solid state fermentation

Several parameters including (a) tray fermentation with and without perforation (b) thickness of solid substrate bed (c) type of inoculum (d) size of inoculum (e) effect of relative humidity were studied for the optimum production of Cyclosporin A by solid state fermentation using Tolypocladium sp. The results indicate that while perforations in the trays had no significance on the yield of Cyc A, the other parameters had an influence on the production of Cyc A. The results indicate that under the optimized conditions, Cyc A can be produced in bulk quantities economically.