Wastewater-Based Epidemiology for Community Monitoring of SARS-CoV-2: Progress and Challenges

被引:42
|
作者
Kumblathan, Teresa [1 ]
Liu, Yanming [1 ]
Uppal, Gursharan K. [1 ]
Hrudey, Steve E. [1 ]
Li, Xing-Fang [1 ]
机构
[1] Univ Alberta, Fac Med & Dent, Dept Lab Med & Pathol, Div Analyt & Environm Toxicol, Edmonton, AB T6G 2G3, Canada
来源
ACS ENVIRONMENTAL AU | 2021年 / 1卷 / 01期
基金
加拿大自然科学与工程研究理事会;
关键词
wastewater-based epidemiology; surveillance; SARS-CoV-2; Covid-19; RNA; RT-qPCR; controls; EXTRACTION PROTOCOL; ENZYMATIC LYSIS; ACID; RNA; SURVEILLANCE; QUANTIFY; VIRUSES; BINDING; PROTEIN;
D O I
10.1021/acsenvironau.1c00015
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Wastewater-basedepidemiology (WBE) is useful for the surveillanceof severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) incommunities, complementing clinical diagnostic testing of individuals.In this Review, we summarize recent progress and highlight remainingchallenges in monitoring SARS-CoV-2 RNA in wastewater systems forcommunity and environmental surveillance. Very low concentrationsof viral particles and RNA present in the complicated wastewater andsewage sample matrix require efficient sample processing and sensitivedetection. We discuss advantages and limitations of available methodsfor wastewater sample processing, including collection, separation,enrichment, RNA extraction, and purification. Efficient extractionof the viral RNA and removal of interfering sample matrices are criticalto the subsequent reverse transcription-quantitative polymerase chainreaction (RT-qPCR) for sensitive detection of SARS-CoV-2 in wastewater.We emphasize the importance of implementing appropriate controls andmethod validation, which include the use of surrogate viruses forassessing extraction efficiency and normalization against measurablechemical and biological components in wastewater. Critical analysisof the published studies reveals imperative research needs for thedevelopment, validation, and standardization of robust and sensitivemethods for quantitative detection of viral RNA and proteins in wastewaterfor WBE.
引用
收藏
页码:18 / 31
页数:14
相关论文
共 50 条
  • [21] Wastewater-Based Epidemiology of SARS-CoV-2 RNA in Bethlehem, PA and Lehigh University
    Alqasawah, Nusaibah
    Ware, Vassie
    Jellison, Kristen
    ENVIRONMENTS, 2024, 11 (10)
  • [22] Surveillance of SARS-CoV-2 spread using wastewater-based epidemiology: Comprehensive study
    Hemalatha, Manupati
    Kiran, Uday
    Kuncha, Santosh Kumar
    Kopperi, Harishankar
    Gokulan, C.G.
    Mohan, S. Venkata
    Mishra, Rakesh K.
    Science of the Total Environment, 2021, 768
  • [23] Contribution of wastewater-based epidemiology to SARS-CoV-2 screening in Brazil and the United States
    Henriques, Taciane Barbosa
    Cassini, Servio Tulio
    de Pinho Keller, Regina
    JOURNAL OF WATER AND HEALTH, 2023, 21 (03) : 343 - 353
  • [24] Surveillance of SARS-CoV-2 spread using wastewater-based epidemiology: Comprehensive study
    Hemalatha, Manupati
    Kiran, Uday
    Kuncha, Santosh Kumar
    Kopperi, Harishankar
    Gokulan, C. G.
    Mohan, S. Venkata
    Mishra, Rakesh K.
    SCIENCE OF THE TOTAL ENVIRONMENT, 2021, 768
  • [25] Wastewater-Based Epidemiology of SARS-CoV-2: Assessing Prevalence and Correlation with Clinical Cases
    Hima Wani
    Smita Menon
    Dipen Desai
    Nishita D’Souza
    Zarine Bhathena
    Nishith Desai
    Joan B. Rose
    Sandhya Shrivastava
    Food and Environmental Virology, 2023, 15 : 131 - 143
  • [26] SARS-CoV-2 variant trends in Ireland: Wastewater-based epidemiology and clinical surveillance
    Reynolds, Liam J.
    Gonzalez, Gabriel
    Sala-Comorera, Laura
    Martin, Niamh A.
    Byrne, Alannah
    Fennema, Sanne
    Holohan, Niamh
    Kuntamukkula, Sailusha Ratnam
    Sarwar, Natasha
    Nolan, Tristan M.
    Stephens, Jayne H.
    Whitty, Megan
    Bennett, Charlene
    Luu, Quynh
    Morley, Ursula
    Yandle, Zoe
    Dean, Jonathan
    Joyce, Eadaoin
    O'Sullivan, John J.
    Cuddihy, John M.
    McIntyre, Angeline M.
    Robinson, Eve P.
    Dahly, Darren
    Fletcher, Nicola F.
    Carr, Michael
    De Gascun, Cillian
    Meijer, Wim G.
    SCIENCE OF THE TOTAL ENVIRONMENT, 2022, 838
  • [27] Integrating Wastewater-Based Epidemiology and Mobility Data to Predict SARS-CoV-2 Cases
    Schenk, Hannes
    Arabzadeh, Rezgar
    Dabiri, Soroush
    Insam, Heribert
    Kreuzinger, Norbert
    Buchel-Marxer, Monika
    Markt, Rudolf
    Nagele, Fabiana
    Rauch, Wolfgang
    ENVIRONMENTS, 2024, 11 (05)
  • [28] An alternative approach for bioanalytical assay optimization for wastewater-based epidemiology of SARS-CoV-2
    Boogaerts, Tim
    Jacobs, Lotte
    De Roeck, Naomi
    Van den Bogaert, Siel
    Aertgeerts, Bert
    Lahousse, Lies
    van Nuijs, Alexander L. N.
    Delputte, Peter
    SCIENCE OF THE TOTAL ENVIRONMENT, 2021, 789
  • [29] Analysis of sampling strategies for pulse loads of SARS-CoV-2: implications for wastewater-based epidemiology
    Koenig, Albert Wilhelm
    Ariano, Sarah Sydney
    Joksimovic, Darko
    WATER SCIENCE AND TECHNOLOGY, 2023, 88 (04) : 1039 - 1057
  • [30] Translating SARS-CoV-2 wastewater-based epidemiology for prioritizing mass vaccination: a strategic overview
    Dharmadhikari, Tanmay
    Yadav, Rakeshkumar
    Dastager, Syed
    Dharne, Mahesh
    ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2021, 28 (31) : 42975 - 42980