Gene expression profiling in skeletal muscle using a dedicated microarray

被引：0
|
作者：
Campanaro, S
Romualdi, C
De Pittà, C
Fanin, M
Celegato, B
Pacchioni, B
Trevisan, S
Laveder, P
Toppo, S
Cagnin, S
Valle, G
Angelini, C
Lanfranchi, G
机构：
[1] Univ Padua, CRIBI, Ctr Biotechnol, I-35131 Padua, Italy
[2] Univ Padua, Dipartimento Biol, I-35131 Padua, Italy
[3] Univ Padua, Dept Psychiat & Neurol Sci, I-35131 Padua, Italy
来源：
MINERVA BIOTECNOLOGICA | 2002年 / 14卷 / 3-4期
关键词：
DNA microarray; gene expression; skeletal muscle; subtraction library; muscular dystrophies;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Background Microarrays can measure simultaneously the expression of thousands of genes and identify subtle changes in expression between different biological states. in our lab a novel two-step subtractive strategy was settled in order to enrich for genes expressed at low levels in skeletal muscle. Methods. Using this cDNA collection we have developed a dedicated muscle-specific microarray composed of 2,700 sequences corresponding to the 400-500 most 3'-terminal region of muscle transcripts. Results. The expression profile of limb-girdle dystrophy type 2B was analysed with our array. Conclusions. We found approximately 3% differentially expressed genes, mostly involved in muscle contraction and immune response.
引用
收藏
页码：273 / 279
页数：7
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