Stable expression of a thermostable xylanase of Clostridium thermocellum in cultured tobacco cells

被引：0

作者：

Kimura, T ^{[1
]}

Mizutani, T ^{[1
]}

Sakka, K ^{[1
]}

Ohmiya, K ^{[1
]}

机构：

[1] Mie Univ, Fac Bioresources, Tsu, Mie 5148507, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2003年 / 95卷 / 04期

关键词：

Clostridium thermocellum; tobacco BY-2; xylanase;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two distinct domains of the xynA gene from Clostridium thermocellum encoding a xylanase catalytic domain (XynA1) and a xylanase catalytic domain with a cellulose binding domain (XynA2) under the control of the cauliflower mosaic virus 35S promoter were electroporated into cultured tobacco BY-2 cells. Transgenic BY-2 calli expressing xylan-hydrolyzing activity were obtained at high frequency for both genes. Western blot analysis using an anti-XynA antibody indicated that XynA1 and XynA2 were produced in these calli.

引用

页码：397 / 400

页数：4

共 50 条

[41] PRODUCTION, PURIFICATION, AND PROPERTIES OF THERMOSTABLE XYLANASE FROM CLOSTRIDIUM-STERCORARIUM
BERENGER, JF
FRIXON, C
BIGLIARDI, J
CREUZET, N
CANADIAN JOURNAL OF MICROBIOLOGY, 1985, 31 (07) : 635 - 643
[42] Expression and display of a novel thermostable esterase from Clostridium thermocellum on the surface of Bacillus subtilis using the CotB anchor protein
Chen, Huayou
Zhang, Tianxi
Jia, Jinru
Vastermark, Ake
Tian, Rui
Ni, Zhong
Chen, Zhi
Chen, Keping
Yang, Shengli
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2015, 42 (11) : 1439 - 1448
[43] Regulation of major cellulosomal endoglucanases of Clostridium thermocellum differs from that of a prominent cellulosomal xylanase
Dror, TW
Rolider, A
Bayer, EA
Lamed, R
Shoham, Y
JOURNAL OF BACTERIOLOGY, 2005, 187 (07) : 2261 - 2266
[44] IDENTIFICATION OF 3 DISTINCT CLOSTRIDIUM-THERMOCELLUM XYLANASE GENES BY MOLECULAR-CLONING
MACKENZIE, CR
YANG, RCA
PATEL, GB
BILOUS, D
NARANG, SA
ARCHIVES OF MICROBIOLOGY, 1989, 152 (04) : 377 - 381
[45] EXPRESSION OF HUMAN ERYTHROPOIETIN IN CULTURED TOBACCO CELLS
MATSUMOTO, S
ISHII, A
IKURA, K
UEDA, M
SASAKI, R
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1993, 57 (08) : 1249 - 1252
[46] NUCLEOTIDE-SEQUENCE AND DELETION ANALYSIS OF THE XYLANASE GENE (XYNZ) OF CLOSTRIDIUM-THERMOCELLUM
GREPINET, O
CHEBROU, MC
BEGUIN, P
JOURNAL OF BACTERIOLOGY, 1988, 170 (10) : 4582 - 4588
[47] The nature of the carbohydrate binding module determines the catalytic efficiency of xylanase Z of Clostridium thermocellum
Khan, Muhammad Imran M.
Sajjad, Muhammad
Sadaf, Saima
Zafar, Rehan
Niazi, Umer H. K.
Akhtar, Muhammad Waheed
JOURNAL OF BIOTECHNOLOGY, 2013, 168 (04) : 403 - 408
[48] Determination of immunological homology between cellulosome subunits and cloned endoglucanases and xylanase of Clostridium thermocellum
Bhat, S
Hutson, RA
Owen, E
Bhat, MK
ANAEROBE, 1997, 3 (05) : 347 - 352
[49] Creation of thermostable and alkaline stable xylanase variants by DNA shuffling
Stephens, Dawn Elizabeth
Khan, Faez Iqbal
Singh, Parvesh
Bisetty, Krishna
Singh, Suren
Permaul, Kugen
Journal of Biotechnology, 2014, 187 : 139 - 146
[50] Creation of thermostable and alkaline stable xylanase variants by DNA shuffling
Department of Biotechnology and Food Technology, Durban University of Technology, ML Sultan Campus, Durban, South Africa
不详
不详
J. Biotechnol., (139-146):

← 1 2 3 4 5 →