Cruciferous vegetable intake questionnaire improves cruciferous vegetable intake estimates

Objective To develop a validated, focused Cruciferous Vegetable Food Frequency Questionnaire (FFQ) as an assessment tool for specific quantification of dietary cruciferous vegetable exposure. Design/methods Participants (n = 107; 18 to 76 years old) completed a standard FFQ and the Cruciferous Vegetable FFQ twice over a 2-week period. Repeat dietary recalls were collected on 3 days over the same 2-week period. Urinary dithiocarbamate was determined as a biomarker of cruciferous vegetable intake. Statistical analyses Descriptive statistics of intake; paired t tests and sign tests for comparison of intake estimates between instruments; Spearman correlations to assess reliability and associations between diet instruments and urinary dithiocarbamate. Results Cruciferous vegetable intake was significantly correlated between the two FFQs (r(s) = 0.58), although the Cruciferous Vegetable FFQ estimated intake 35 g higher than the standard FFQ. The Cruciferous Vegetable FFQ was reliable, with a repeated measures correlation of 0.69 (P <= 0.01). Urinary dithiocarbamate excretion correlated with cruciferous vegetable intake from the Cruciferous Vegetable FFQ (r(s) = 0.26, P < 0.01), and from the standard FFQ (r(s) = 0.19, P = 0.06). Conclusions The Cruciferous Vegetable FFQ provided a reproducible, valid estimate of cruciferous vegetable exposure and improved the relationship between crucifer consumption and urinary dithiocarbamate, a biomarker of cruciferous vegetable exposure'. The nearly twofold difference in exposure estimates between the Cruciferous Vegetable FFQ and the standard FFQ could change the statistical significance of risk estimates in the context of epidemiological research. This questionnaire is an appropriate research tool to evaluate cruciferous vegetable intake more accurately than a standard FFQ, particularly in the context of dietary intervention studies that promote increased vegetable intake to reduce the risk for chronic disease.