Stimulation-induced responses of the trigeminal caudal neurons in the brainstem preparation isolated from newborn rats

被引:11
|
作者
Hamba, M [1 ]
机构
[1] Showa Univ, Sch Dent, Dept Physiol, Shinagawa Ku, Tokyo 142, Japan
关键词
pain; windup; development; slow; excitatory postsynaptic potential (EPSP); N-methyl-D-aspartate (NMDA) receptor;
D O I
10.1016/S0006-8993(97)01382-6
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The brainstem preparation with the trigeminal mandibular nerve attached was isolated from rats postnatal day 0-6 (P0-P6) to test if the potentiation could be induced in neonatal neurons in the trigeminal subnucleus caudalis by stimulation of the primary afferents. The stimulation-induced potentials in 92 neurons recorded extracellularly, and the synaptic potentials in 16 neurons recorded by the whole-cell patch clamp technique were examined. The extracellularly recorded neurons responded to stimulation (0.5 Hz) with either an increase, a decrease, or little change in spike numbers, and it ere classified as Type 1, Type 2, and Type 3. respectively. Type 1 neurons at P4 and older responded in a low Mg2+ solution with a progressive increase in spike number lasting for several minutes after the cessation of stimulation, i.e., short-term potentiation, STP. This potentiation was antagonized by 20 mu M of(+)-MK-801 hydrogen maleate (MK-801) or 25 mu M of 2-amino-5-phosphonovaleric acid. In contrast, Type 1 at P3 and younger did not exhibit STP. The age-related distinct response properties were observed between Type 1 neurons at P4-P6 and at P0-P3. The percentage of Type 1 in studied neurons increased from 24% at P0-P3 to 53% at P4-P6. In the intracellular experiment, the mean latency of excitatory postsynaptic potential (EPSP) of recorded neurons indicated that the conduction velocity of the convergent afferents was 0.37 m/s, in the range of C-fiber. Neurons were classified as Type E and Type I. Type E responded with EPSP only, or with both EPSP and inhibitory postsynaptic potentials (IPSP), while Type I responded with IPSP only. In Type E at P4 and older, a single stimulation produced a burst of spike discharges that lasted for several seconds. Stimulation at a hyperpolarized membrane potential showed that aggregated slow EPSPs lay under a burst of spike discharges, and that slow EPSPs, but not a short-latency EPSP, were completely blocked by MK-801. In contrast, Type E at P3 and younger did not evoke a burst of spikes. Morphological examination of recorded neurons showed that the formation of networks was sparse at P4 and rapidly developed up to P4. The results suggest that: (1) short-term potentiation is induced with the development of synaptic network formation in the caudal nucleus at P4 and older, (2) the summation of N-methyl-D-aspartate (NMDA)-mediated slow EPSPs build up a prolonged depolarization: and (3) the brainstem preparation is applicable for neurophysiological studies on the trigeminal pain system. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:66 / 74
页数:9
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