Comparison of infectious haematopoietic necrosis virus (IHNV) isolation on monolayers and in suspended cells

被引：2
|
作者：
Hostnik, P [1 ]
Jencic, V [1 ]
机构：
[1] Fac Vet, Ljubljana 1000, Slovenia
来源：
DISEASES OF AQUATIC ORGANISMS | 2000年 / 40卷 / 03期
关键词：
IHNV; EPC; susceptibility;
D O I：
10.3354/dao040225
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
A cell culture Virus isolation procedure for infectious haematopoietic necrosis virus (IHNV) in the epithelioma papulosum cyprini cell line (EPC) is described. Ovarian fluid samples were collected from fish and tested for IHNV at 9 farms. The samples were inoculated in parallel on 24 h old EPC cell monolayers and in freshly trypsinized cells. The titre of the initial virus isolation and of first passages were compared using the 2 methods for each sample. Titres were consistently higher in suspended cells and this method also proved more sensitive for isolation of IHN virus from ovarian fluids of infected fish.
引用
收藏
页码：225 / 228
页数：4
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