Celeribacter manganoxidans sp nov., a manganese-oxidizing bacterium isolated from deep-sea sediment of a polymetallic nodule province

A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped, manganese-oxidizing bacterial strain, designated DY2-5(T), was isolated from surface sediment of Pacific Clarion-Clipperton Fracture Zone (CCFZ). Growth occurred at 0-37 degrees C (optimum 28 degrees C), pH 6.5-9.0 (optimum pH 7.0-7.5) and in the presence of 1-11 % (w/v) NaCl (optimum 3-4 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the novel strain was most closely related to Celeribacter halophilus ZXM137(T) with 96.13 % sequence similarity, and had 16S rRNA gene sequence similarities in the range 93.89-95.87 % with other species of the genus Celeribacter. The dominant fatty acids were summed feature 8 (C-18 : 1 omega 7c and/or C-18 : 1 omega 6c) and C-16 (:) (0). The polar lipids of strain DY2-5(T) comprised phosphatidylglycerol, phosphatidylcholine and two unknown aminolipids. The major respiratory quinone was ubiquinone-10 (Q-10). The DNA G+C content of strain DY2-5(T) was 64.8 mol%. On the basis of the phenotypic, genotypic and physiological evidence, strain DY2-5(T) represents a novel species of the genus Celeribacter, for which the name Celeribacter manganoxidans sp. nov. is proposed. The type strain is DY2-5(T) (=JCM 19384(T) =KCTC 32473(T)).