To study the water environment status of the Irtysh River, water samples from 11 points of the Irtysh River's trunk stream were collected for three times (spring, summer and autumn) in 2019. Some water indexes. such as permanganate index, total phosphorus, total nitrogen, ammonia nitrogen, petroleum. heavy metal and so on. were tested. Combined with the population density along the Irtysh river and other factors, the influence of human activity on water quality was analyzed. The results showed that in 2019, the pH value ranged from 7.10 to 8.14, and the dissolved oxygen ranged from 3.02 to 10.3 mg/L, reaching a low level in spring and autumn. The hardness ranged from 28,4 to 303.9 mg/L, the alkalinity from 19.21 to 156.5 mg/L, the water suspensions from 0.2 to 73.5 mg/L, and ammonia nitrogen from 0 to 1.210 mg/L. In the lower reaches of the Irtysh River where there are large populated areas such as Altay City, Fuhai County, Burgin County and so on, water quality declined sharply. As this area is densely populated, it can be found out that the fishery environment of the Irtysh River is significantly related to human activity.