Biosurfactants of Lactobacillus helveticus for biodiversity inhibit the biofilm formation of Staphylococcus aureus and cell invasion

被引：21
|
作者：
Jiang, Xinpeng [1 ,2 ]
Yan, Xin [1 ]
Gu, Shanshan [1 ]
Yang, Yan [1 ]
Zhao, Lili [3 ]
He, Xinmiao [2 ]
Chen, Hongyan [3 ]
Ge, Junwei [1 ]
Liu, Di [2 ]
机构：
[1] Northeast Agr Univ, Heilongjiang Key Lab Anim Dis Control & Pharmaceu, Coll Vet Med, Harbin 150030, Heilongjiang, Peoples R China
[2] Heilongjiang Acad Agr Sci, Key Lab Combining Farming & Anim Husb, Minist Agr, Anim Husb Res Inst, Harbin 150086, Heilongjiang, Peoples R China
[3] Chinese Acad Agr Sci, Heilongjiang Prov Key Lab Lab Anim & Comparat Med, State Key Lab Vet Biotechnol, Harbin Vet Res Inst, Harbin 150069, Heilongjiang, Peoples R China
来源：
FUTURE MICROBIOLOGY | 2019年 / 14卷 / 13期
基金：
中国国家自然科学基金;
关键词：
adhesion; biofilm formation; biosurfactants; host cell; Lactobacillus helveticus; Staphylococcus aureus; ACIDOPHILUS-DERIVED BIOSURFACTANT; GENE-EXPRESSION; ADHESION; ANTIADHESIVE; SENSITIVITY; PREVENTION; PLANTARUM; VIRULENCE;
D O I：
10.2217/fmb-2018-0354
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: This study aimed to evaluate the differences of biosurfactants produced by two Lactobacillus helveticus strains against the biofilm formation of Staphylococcus aureus in vitro and in vivo. Materials & methods: Scanning electron microscopy, Real time-quantitative PCR (RT-qPCR) and cell assay were used to analyze the inhibiting effect of biosurfactants against biofilm formation. Results & conclusion: Results showed that the biosurfactants have anti-adhesive and inhibiting effects on biofilm formation in vivo and in vitro. The biofilm-formative genes and autoinducer-2 signaling regulated these characteristics, and the biosurfactant L. helveticus 27170 is better than that of 27058. Host cell adhesion and invasion results indicated that the biosurfactants L. helveticus prevented the S. aureus invading the host cell, which may be a new strategy to eliminate biofilms.
引用
收藏
页码：1133 / 1146
页数：14
相关论文
共 50 条
  • [31] MgrA Represses Biofilm Formation in Staphylococcus aureus
    Trotonda, Maria Pilar
    Tamber, Sandeep
    Memmi, Guido
    Cheung, Ambrose L.
    INFECTION AND IMMUNITY, 2008, 76 (12) : 5645 - 5654
  • [32] Heterologous expression of Staphylococcus aureus sasC in Staphylococcus carnosus confers cell aggregation and biofilm formation
    Schroeder, K.
    Jularic, M.
    Hirschhausen, N.
    Neumann, C.
    Bertling, A.
    Kehrel, B.
    Peters, G.
    Heilmann, C.
    INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2008, 298 : 85 - 85
  • [33] Oligopeptide Sortase Inhibitor Modulates Staphylococcus aureus Cell Adhesion and Biofilm Formation
    Bozhkova, Svetlana A.
    Gordina, Ekaterina M.
    Labutin, Dmitry V.
    Kudryavtsev, Konstantin V.
    ANTIBIOTICS-BASEL, 2022, 11 (12):
  • [34] Inhibition of Staphylococcus aureus biofilm by Lactobacillus isolated from fine cocoa
    Melo, Taua Alves
    dos Santos, Thalis Ferreira
    de Almeida, Milena Evangelista
    Gusmao Fontes Junior, Luiz Alberto
    Andrade, Ewerton Ferraz
    Rezende, Rachel Passos
    Marques, Lucas Miranda
    Romano, Carla Cristina
    BMC MICROBIOLOGY, 2016, 16 : 1 - 9
  • [35] Inhibition of Staphylococcus aureus biofilm by Lactobacillus isolated from fine cocoa
    Tauá Alves Melo
    Thalis Ferreira dos Santos
    Milena Evangelista de Almeida
    Luiz Alberto Gusmão Fontes Junior
    Ewerton Ferraz Andrade
    Rachel Passos Rezende
    Lucas Miranda Marques
    Carla Cristina Romano
    BMC Microbiology, 16
  • [36] Calcium-chelating alizarin and other anthraquinones inhibit biofilm formation and the hemolytic activity of Staphylococcus aureus
    Jin-Hyung Lee
    Yong-Guy Kim
    Shi Yong Ryu
    Jintae Lee
    Scientific Reports, 6
  • [37] Extracellular Vehicles from Commensal Skin Malassezia restricta Inhibit Staphylococcus aureus Proliferation and Biofilm Formation
    Liu, Xin
    Guo, Xiaoyu
    Su, Xiaomin
    Ji, Bingru
    Chang, Yawei
    Huang, Qichao
    Zhang, Yuan
    Wang, Xiaobing
    Wang, Pan
    ACS INFECTIOUS DISEASES, 2024, 10 (02): : 624 - 637
  • [38] Clinically Approved Drugs Inhibit the Staphylococcus aureus Multidrug NorA Efflux Pump and Reduce Biofilm Formation
    Zimmermann, Saskia
    Klinger-Strobel, Mareike
    Bohnert, Juergen A.
    Wendler, Sindy
    Roedel, Juergen
    Pletz, Mathias W.
    Loeffler, Bettina
    Tuchscherr, Lorena
    FRONTIERS IN MICROBIOLOGY, 2019, 10
  • [39] Calcium-chelating alizarin and other anthraquinones inhibit biofilm formation and the hemolytic activity of Staphylococcus aureus
    Lee, Jin-Hyung
    Kim, Yong-Guy
    Ryu, Shi Yong
    Lee, Jintae
    SCIENTIFIC REPORTS, 2016, 6
  • [40] Evaluating the effect of Staphylococcus epidermidis proteases on Staphylococcus aureus biofilm formation
    Cao, A.
    Bhattacharya, M.
    Keim, K.
    Horswill, A.
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2024, 144 (08) : S172 - S172
12345