Effect of boron on microspore embryogenesis and direct embryo to plant conversion in Brassica napus (L.)

被引：8

作者：

Mahasuk, Pitchayapa ^{[1
]}

Kullik, Annika Stina ^{[1
]}

Iqbal, Mohammed Cassim ^{[2
]}

Mollers, Christian ^{[1
]}

机构：

[1] Univ Gottingen, Dept Crop Sci, Von Siebold Str 8, D-37075 Gottingen, Germany

[2] Natl Inst Fundamental Studies, Plant & Environm Sci, Hantana Rd, Kandy 20000, Sri Lanka

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2017年 / 130卷 / 02期

关键词：

Boron; Stress; Reactive oxygen species; ROS; Microspore embryogenesis; Direct embryo to plant conversion; Brassica napus; IN-VITRO; CULTURES; INDUCTION; REGENERATION; DEFICIENCY; SULFATE; POLLEN;

D O I：

10.1007/s11240-017-1232-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The NLN-medium has been successfully used, since 1982, for microspore culture in Brassica napus and other Brassica species. Changes to the media composition were restricted to carbohydrate and nitrogen sources and growth regulators while micro-nutrients have not been optimized. The NLN-medium contains boron at a concentration of 162 mu M. Boron is required for diverse physiological and metabolic processes in the cell. This study investigated the effect of seven- and 13-fold increased boron concentration on the induction of embryos in microspore cultures of four genotypes of B. napus. A significant improvement of microspore embryogenesis was achieved by both enhanced boron concentrations in the NLN medium. No effect on the regeneration of embryo to plant conversion was observed.

引用

页码：443 / 447

页数：5

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