Metabolomics analysis I. Selection of biological samples and practical aspects preceding sample preparation

被引：112

作者：

Alvarez-Sanchez, B.

Priego-Capote, F. ^{[1
]}

Luque de Castro, M. D.

机构：

[1] Univ Cordoba, Dept Analyt Chem, E-14071 Cordoba, Spain

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2010年 / 29卷 / 02期

关键词：

Analytical criteria; Biological sample; Metabolite; Metabolomics; Sample preparation; Sample selection; Sample storage; HUMAN CEREBROSPINAL-FLUID; FUNCTIONAL GENOMICS; SYSTEMS BIOLOGY; HUMAN URINE; CAPILLARY-ELECTROPHORESIS; SACCHAROMYCES-CEREVISIAE; LIQUID-CHROMATOGRAPHY; AMNIOTIC-FLUID; EXTRACTION; STABILITY;

D O I：

10.1016/j.trac.2009.12.003

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Metabolomics is one of the most recently emerged "-omics" sciences. Its significance in systems biology is gaining interest to levels similar to proteomics, transcriptomics and genomics. One of the main limitations in metabolomics analysis is the lack of totally comprehensive approaches and in-depth studies, as individuals or laboratories with different skill sets usually develop these. This variability particularly affects sample preparation due to the extensive heterogeneity of biological samples. This two-part review aims to offer a practical guide to preparing biological samples in metabolornics in order to reach the current level in genomics and proteomics. The first part focuses on analytical criteria for sample selection and operations that precede sample preparation. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：111 / 119

页数：9

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