Glutamate regulates the frequency of spontaneous synchronized Ca2+ spikes through group II metabotropic glutamate receptor in cultured mouse cortical networks

被引:3
|
作者
Yasumoto, F
Negishi, T
Ishii, Y
Kyuwa, S
Kuroda, Y
Yoshikawa, Y
机构
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biomed Sci, Bunkyo Ku, Tokyo 1138657, Japan
[2] Tokyo Metropolitan Inst Neurosci, Dept Mol & Cellular Neurobiol, Fuchu, Tokyo, Japan
[3] Japan Sci & Technol Corp, Core Res Evolut Sci & Technol, Kawaguchi, Saitama, Japan
关键词
mGluRs; PKA; synaptic transmission; Ca2+ imaging; synchronized Ca(2+)spikes;
D O I
10.1007/s10571-004-6923-x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
1. Synchronized spontaneous intracellular Ca2+ spikes in networked neurons are believed to play a major role in the development and plasticity of neural circuits. Glutamate-induced signals through the ionotropic glutamate receptors (iGluRs) are profoundly involved in the generation of synchronized Ca2+ spikes. 2. In this study, we examined the involvement of metabotropic glutamate receptors (mGluRs) in cultured mouse cortical neurons. We pharmacologically revealed that glutamate-induced signals through inclusive mGluRs decreased the frequency of Ca2+ spikes. Further experiments indicated that this suppressive effect on the spike frequency was mainly due to the signal through group II mGluR, inactivation of adenylate cyclase-cAMP-PKA signaling pathway. Group I mGluR had little involvement in the spike frequency. 3. Taken together, glutamate generates the synchronized Ca2+ spikes through iGluRs and modulates simultaneously their frequency through group II mGluR-adenylate cyclase-cAMP-PKA signaling pathway in the present in vitro neural network. These results provide the evidence of the profound role of group II mGluR in the spontaneous and synchronous neural activities.
引用
收藏
页码:841 / 852
页数:12
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