Potential misidentification of Burkholderia pseudomallei by API 20NE

被引:53
|
作者
Inglis, TJJ
Chiang, D
Lee, GSH
Chor-Kiang, L
机构
[1] Natl Univ Singapore, Dept Microbiol, Singapore 117548, Singapore
[2] Natl Univ Singapore Hosp, Dept Lab Med, Singapore 117548, Singapore
关键词
Burkholderia pseudomallei; API; 20NE; melioidosis;
D O I
10.1080/00313029800169685
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Biochemical confirmation of the identity of Burkholderia pseudomallei in Singapore previously relied on the API 20NE panel of tests. After introducing an alternative proprietary biochemical panel, the Microbact 24E (MedVet, Adelaide, Australia), we noted that the API panel identified some presumptive B. pseudomallei isolates as other species. We therefore compared the performance of the API 20NE against the Microbact 24E with 50 distinct clinical isolates of B. pseudomallei, after 24 hours and after five days incubation of primary cultures. The API panel correctly identified 40 isolates, Four results were unacceptable or uninterpretable. Six isolates were misidentified as other species; the commonest being Chromobacterium violaceum. One of these was again identified as C. violaceum by the repeat API panel. Fourteen isolates, including the six misidentified isolates and four isolate pairs from separate sources in four separate patients, were typed using PCR amplification of repetitive extragenic palindromic sequences (REPS). The isolates identified as C, violaceum appeared to have identical REPS patterns, suggesting that some of the errant API results may be due to a single locally prevalent strain of B. pseudomallei. A previous suggestion that C. violaceum may produce a melioidosis-like illness may therefore be due to laboratory misidentification of B, pseudomallei with the API 20NE biochemical test panel.
引用
收藏
页码:62 / 64
页数:3
相关论文
共 50 条
  • [21] Anatomy of molecular structures in 20Ne
    Zhou, E. F.
    Yao, J. M.
    Li, Z. P.
    Meng, J.
    Ring, P.
    PHYSICS LETTERS B, 2016, 753 : 227 - 231
  • [22] The 5α condensate state in 20Ne
    Zhou, Bo
    Funaki, Yasuro
    Horiuchi, Hisashi
    Ma, Yu-Gang
    Roepke, Gerd
    Schuck, Peter
    Tohsaki, Akihiro
    Yamada, Taiichi
    NATURE COMMUNICATIONS, 2023, 14 (01)
  • [23] The fragmentation of 20Ne at 400 A MeV
    程锦霞
    张东海
    晏世伟
    李俊生
    王立春
    李彦晶
    N.Yasuda
    中国物理C, 2012, 36 (01) : 37 - 42
  • [24] Unnecessary level in the 20Ne nucleus
    Artemov, KP
    Golovkov, MS
    Pankratov, VV
    Rudakov, VP
    PHYSICS OF ATOMIC NUCLEI, 1999, 62 (07) : 1154 - 1157
  • [25] The 5α condensate state in 20Ne
    Takahiro Kawabata
    Nuclear Science and Techniques, 2024, 35
  • [26] The 5α condensate state in 20Ne
    Kawabata, Takahiro
    NUCLEAR SCIENCE AND TECHNIQUES, 2024, 35 (02)
  • [27] The fragmentation of 20Ne at 400 A MeV
    Cheng Jin-Xia
    Zhang Dong-Hai
    Yan Shi-Wei
    Li Jun-Sheng
    Wang Li-Chun
    Li Yan-Jing
    Yasuda, N.
    CHINESE PHYSICS C, 2012, 36 (01) : 37 - 42
  • [28] The 5α condensate state in 20Ne
    Bo Zhou
    Yasuro Funaki
    Hisashi Horiuchi
    Yu-Gang Ma
    Gerd Röpke
    Peter Schuck
    Akihiro Tohsaki
    Taiichi Yamada
    Nature Communications, 14
  • [29] EFFECTS OF PAIRING IN 20NE NUCLEUS
    KALMAN, CS
    BERNIER, JP
    HARVEY, M
    CANADIAN JOURNAL OF PHYSICS, 1967, 45 (03) : 1297 - &
  • [30] Initial State Interaction for the 20Ne
    Carbone, Diana
    Linares, Roberto
    Amador-Valenzuela, Paulina
    Calabrese, Salvatore
    Cappuzzello, Francesco
    Cavallaro, Manuela
    Firat, Suna
    Fisichella, Maria
    Spatafora, Alessandro
    Acosta, Luis
    Agodi, Clementina
    Boztosun, Ismail
    Brischetto, Giuseppe A.
    Calvo, Daniela
    Chavez Lomeli, Efrain R.
    Ciraldo, Irene
    Cutuli, Mauro
    Delaunay, Franck
    Deshmukh, Nikit
    Finocchiaro, Paolo
    Foti, Antonino
    Hacisalihoglu, Aylin
    Iazzi, Felice
    La Fauci, Laura
    Lanzalone, Gaetano
    Medina, Nilberto H.
    Mendes, Djalma
    Oliveira, Jose R. B.
    Pakou, Athina
    Pandola, Luciano
    Petrascu, Horia
    Pinna, Federico
    Russo, Giuseppe
    Sgouros, Onoufrios
    Solakci, Selcuk O.
    Soukeras, Vasilis
    Souliotis, George
    Torresi, Domenico
    Tudisco, Salvatore
    Yildirim, Aydin
    Zagatto, Vinicius A. B.
    UNIVERSE, 2021, 7 (03)