New methods for measuring 1H-31P coupling constants in nucleic acids

被引：0

作者：

Gotfredsen, CH ^{[1
]}

Meissner, A ^{[1
]}

Duus, JO ^{[1
]}

Sorensen, OW ^{[1
]}

机构：

[1] Carlsberg Lab, Dept Chem, DK-2500 Valby, Denmark

来源：

MAGNETIC RESONANCE IN CHEMISTRY | 2000年 / 38卷 / 08期

关键词：

H-1-P-13 coupling constants; multidimensional NMR; DNA duplex; J-HMBC; scaling;

D O I：

10.1002/1097-458X(200008)38:8<692::AID-MRC723>3.0.CO;2-O

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

New pulse sequences for the measurement of H-1-->P-31 coupling constants in the backbone of DNA duplexes are presented. They have a resemblance to HMBC but the constant J dephasing delay is exploited for heteronuclear J modulation so that the peaks in the two-dimensional spectrum appear as doublets with splittings scaled relative to the P-31 chemical shifts. The new pulse sequences have been applied to a self-complementary octamer DNA duplex 5'-CGCTAGCG-3' (CTAG). Copyright (C) 2000 John Wiley & Sons, Ltd.

引用

页码：692 / 695

页数：4

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