New methods for measuring 1H-31P coupling constants in nucleic acids

被引：0
|
作者：
Gotfredsen, CH [1 ]
Meissner, A [1 ]
Duus, JO [1 ]
Sorensen, OW [1 ]
机构：
[1] Carlsberg Lab, Dept Chem, DK-2500 Valby, Denmark
来源：
MAGNETIC RESONANCE IN CHEMISTRY | 2000年 / 38卷 / 08期
关键词：
H-1-P-13 coupling constants; multidimensional NMR; DNA duplex; J-HMBC; scaling;
D O I：
10.1002/1097-458X(200008)38:8<692::AID-MRC723>3.0.CO;2-O
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
New pulse sequences for the measurement of H-1-->P-31 coupling constants in the backbone of DNA duplexes are presented. They have a resemblance to HMBC but the constant J dephasing delay is exploited for heteronuclear J modulation so that the peaks in the two-dimensional spectrum appear as doublets with splittings scaled relative to the P-31 chemical shifts. The new pulse sequences have been applied to a self-complementary octamer DNA duplex 5'-CGCTAGCG-3' (CTAG). Copyright (C) 2000 John Wiley & Sons, Ltd.
引用
收藏
页码：692 / 695
页数：4
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