A novel antigen delivery system induces strong humoral and CTL immune responses

被引：29
|
作者：
Yang, Zishan [1 ,2 ]
Xu, Miaomiao [1 ,2 ]
Jia, Zhenghu [1 ,2 ]
Zhang, Yuting [1 ,2 ]
Wang, Li [1 ,2 ]
Zhang, Hongru [1 ,2 ]
Wang, Jingya [1 ,2 ]
Song, Mei [1 ,2 ]
Zhao, Yapu [4 ]
Wu, Zhenzhou [1 ,2 ]
Zhao, Liqing [1 ,2 ]
Yin, Zhinan [3 ]
Hong, Zhangyong [1 ,2 ]
机构：
[1] Nankai Univ, Natl Key Lab Med Chem Biol, Room C-203,Room A-407,Biol Stn, Tianjin 300071, Peoples R China
[2] Nankai Univ, Tianjin Key Lab Prot Sci, Room C-203,Room A-407,Biol Stn, Tianjin 300071, Peoples R China
[3] Jinan Univ, Biomed Translat Res Inst, 10th Floor Off,Sch Educ Bldg, Guangzhou 510632, Guangdong, Peoples R China
[4] Peoples Liberat Army Hosp 254, Biotherapy Ctr, Tianjin 300142, Peoples R China
来源：
BIOMATERIALS | 2017年 / 134卷
基金：
中国国家自然科学基金; 国家高技术研究发展计划(863计划);
关键词：
Glucan particles; Chitosan; CTL response; Adjuvant; Dendritic cells; FUNGAL BETA-GLUCANS; DENDRITIC CELLS; RECEPTOR; DECTIN-1; CANCER; IMMUNOTHERAPY; DIFFERENTIATION; STIMULATION; RECOGNITION; INDUCTION;
D O I：
10.1016/j.biomaterials.2017.04.035
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
New strategies with the ability to enhance both the humoral and cellular immune responses remain a priority for the development of future therapeutic cancer vaccines. In this study, we took advantage of beta-glucan particles (GPs) derived from Saccharomyces cerevisiae baker's yeast and a novel reverse microemulsion method to prepare an antigen-loaded GP carrier system for dendritic cell (DC) specific antigen delivery, followed by careful evaluation of the immune functions of the prepared particles in initiating both the humoral and cellular immune responses through in vitro and in vivo experiments. The prepared particles greatly promoted DC activation and cytokine production and cross presented the antigen to CD8 cells, inducing very strong OVA specific humoral and cellular immune responses. Treatment with these particles significantly prevented the growth of implanted EG7-OVA tumors in a prophylactic and pre-established tumor model. These results suggest that our strategy may be able to be utilized as a promising platform for cancer immunotherapy. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码：51 / 63
页数：13
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