Different physiognomies across the Cerrado could influence the microbial C:N:P ratio in the soil since these physiognomies present different abundance and diversity of plant species. Thus, the aim of this study was to evaluate the microbial C:N:P ratio in soil across three different physiognomies of Cerrado in the Northeast, Brazil, namely campo graminoide (dominance of grasses), cerrado stricto sensu (dominance of grasses, shrubs, low trees, and woody stratum), and cerradao (dominance of woody stratum). Campo graminoide was characterized by lower values of total organic C, N, microbial C:P, N:P, and soil C:N. Cerrado stricto sensu presented average values for most of the measured parameters, while cerradao presented higher values of microbial C, N, P, organic C, N and soil C:P and C:N ratios. The principal component analysis showed that the samples grouped according to the sites, with a clear gradient from campo graminoide to cerradao. Therefore, the differences of vegetation across physiognomies of Cerrado influenced the soil microbial C:N:P ratio, where cerradao showed highest microbial C:N:P ratio than soil under campo graminoide.