Production, characterization and growth inhibitory potential of metabolites produced by Pseudomonas and Bacillus species

A variety of metabolites obtained from microbes are indicated to have anti-inflammatory, anticancer and antibiotics activities. This study was therefore aimed at assessing the antibacterial potentials of metabolites produced by four bacterial species against selected pathogens. The study was carried out under batch conditions. A 24 h old culture of each bacteria species was inoculated into 200 mL medium in 250 mL capacity conical flasks and incubated. The metabolites showed antimicrobial activity against the selected typed and clinical isolates. In presence of the respective crude metabolites, growth of Salmonella typhi and Klebsiella pneumoniae were observed to be inhibited during the period of incubation, except for setup that contained metabolites produced by the Bacillus cereus. The metabolite combinations from P. aeruginosa and P. fuscoginae, P. aeruginosa and B. subtilis and from P. aeruginosa and B. cereus were observed to inhibit most of the isolates. Structural characterization of the metabolites detected the presence of peptide-like moiety, as well as aliphatic hydrocarbons. The antimicrobial potential shown by the metabolites is a promising indication for further studies to be carried out with respect to possible safe use of the purified metabolites as potential antimicrobials in humans and animals. (C) 2021 The Author(s). Published by Elsevier B.V. on behalf of African Institute of Mathematical Sciences / Next Einstein Initiative.