Attenuation of ischemia-reperfusion injury with Marrubium cordatum treatment in ovarian torsion-detorsion model in rabbits

Objective: To investigate protective effects of Marrubium cordatum extract on ovary torsion-detorsion. Design: Controlled research study. Setting: Marrubium cordatum extract was obtained by methanol extraction. Animal(s): Six-month-old female New Zealand rabbits. Intervention(s): In the first phase, antioxidant activity of M. cordatum extract was evaluated. In the second phase, M. cordatum extract at doses of 0, 250, 500, and 1,000 mg/kg was studied for dose determination. In the third phase, the protective role of M. cordatum on ovarian torsion detorsion injury was evaluated in sham control, torsion detorsion, torsion-detorsion + M. cordatum (1,000 mg/kg). Main Outcome Measure(s): 1,1-Dipheny1-2-picrylhydrazyl, nitric oxide radical scavenging activity, reducing power capacity, and total phenolic compounds were assayed. Glutathione, malondialdehyde, catalase, and glutathione peroxidase were measured. Histopathological examination was also conducted. Result(s): Marrubium cordatum significantly inhibited 1,1-diphenyl-2-picrylhydrazyl, nitric oxide radicals, and showed a powerful reducing activity. Marrubium cordatum did not adversely affect biochemical and histopathological parameters at all doses. Malondialdehyde level and catalase activity in the torsion detorsion group were significantly increased compared with those of the sham group, whereas the glutathione level and glutathione peroxidase activity were significantly decreased compared with those of the sham group. Marrubium cordatum treatment significantly lowered the malondialdehyde level and catalase activity but increased the glutathione level in torsion-detorsion injury. Histopathologically, severe congestion, hemorrhage, edema, and leukocyte infiltration were observed in the torsion detorsion group. Marrubium cordatum treatment ameliorated these alterations. Conclusion(s): Marrubium cordatum attenuates ischemia-reperfusion-induced biochemical and histopathological alterations. (Fertil Steril (R) 2010;93:1455-63. (C)2010 by American Society for Reproductive Medicine.)