Shiga toxin B-subunit sequential binding to its natural receptor in lipid membranes

被引:21
|
作者
Pina, David G.
Johannes, Ludger
Castanho, Miguel A. R. B.
机构
[1] Univ Lisbon, Fac Ciencias, Ctr Quim & Bioquim, P-1749016 Lisbon, Portugal
[2] Inst Curie, CNRS, UMR 144, Lab Traf & Signalisat, F-75248 Paris 05, France
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2007年 / 1768卷 / 03期
关键词
Shiga toxin B-subunit; globotriaosyl ceramide; fluorescence; lipid vesicles;
D O I
10.1016/j.bbamem.2006.12.011
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Shiga toxin B-subunit (STxB), a protein involved in the cell-binding and intracellular trafficking of Shiga holotoxin, binds to a specific glycolipid, the globotriaosyl ceramide (Gb(3)) Tryptophan residues of STxB, located at the protein-membrane interface, allow one to study its interaction with model membranes by means of spectroscopic methods with no need for chemical derivatisation with a fluorophore. The protein emits maximally around 346 nm and a blue shift of about 8 mn, as well as the occurrence of changes in the emission fluorescence intensity spectra, is indicative of insertion and partition into the membrane. However, the interaction seems to take place without pentamer dissociation. Acrylamide quenching experiments confirm tryptophan residues become less exposed to solvent when in the presence of vesicles, and the use of lipophilic probes suggests that they are located in a shallow position near the water/membrane interface. Fluorescence intensity and lifetime measurements upon STxB titration with Gb(3)-containing vesicles suggest a complex STxB/Gb(3) docking mechanism involving static quenching in the later stages. Based on our observations, a model of the protein-membrane interaction is proposed and the STxB membrane partition and binding constants were calculated. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:628 / 636
页数:9
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