Bioassay studies were conducted using the leaf dip method for endosulfan, imidacloprid, acetameprid and diafenthiuron to determine the susceptibility of Bemisia tabaci Gen. from three districts (Bahawalpur, Faisalabad, and Multan) of the Punjab, Pakistan. It was observed that all the B. tabaci populations were susceptible to these pesticides. Comparative resistance ratios of these insecticides at LC(50) were 1.75-3.60 folds for endosulfan, 1.18-2.09 folds for imidacloprid, 1.01-4.29 folds for acetameprid and 1.06-2.0 folds for diafenthiuron. In the present studies, the variability in the tolerance of B. tabaci is measured for the first time against endosulfan, acetameprid and diafenthiuron against Bemisia tabaci Gen. in Pakistan. The wise use of these insecticides for controlling B. tabaci Gen. field populations in the presence of conventional insecticides will help for its better management.