THE SOD2 MUTANT MOUSE AS A MODEL FOR OXIDATIVE STRESS: A FUNCTIONAL PROTEOMICS PERSPECTIVE

Oxidative stress has been implicated ill the pathogenesis of numerous human diseases diseases mid disorders, but the mechanistic basis often remains enigmatic. The Sod2 mutant mouse. which is sensitized to mitochondrial stress, is an ideal mutant model for studying the role of oxidative stress in a diverse range of complications arising from mitochondrial dysfunction and diminished antioxidant defense. To fully appreciate the wide-spread molecular consequences under increased oxidative stress, a systems approach utilizing proteomics is able to provide a global overview of the complex biological changes, which a targeted single biomolecular approach cannot address fully.. This review focuses oil the applications of moss spectrometry mid functional proteomics in the Sod2 mouse. The combinatorial approach provides novel insights into the interplay of chemistry and biology, free radicals and proteins, thereby augmenting our understanding of how redox perturbations influence protein dynamics. Ultimately, this knowledge can lead to the development of free radical-targeted therapies. (C) 2009 Wiley Periodicals, Inc., Mass Spec Rev 29: 179-196, 2010