Molecular Cloning and Characterization of Rubber Biosynthetic Genes from Taraxacum koksaghyz

被引：91
|
作者：
Schmidt, Thomas [1 ]
Hillebrand, Andrea [1 ]
Wurbs, David [1 ]
Wahler, Daniela [1 ]
Lenders, Malte [1 ]
Gronover, Christian Schulze [2 ]
Pruefer, Dirk [1 ,2 ]
机构：
[1] Univ Munster, Inst Biochem & Biotechnol Pflanzen, D-48143 Munster, Germany
[2] Fraunhofer Inst Mol & Angew Okol, D-52074 Aachen, Germany
来源：
PLANT MOLECULAR BIOLOGY REPORTER | 2010年 / 28卷 / 02期
关键词：
Taraxacum koksaghyz; Rubber; Latex; Laticifers; Rubber particles; HEVEA-BRASILIENSIS; CIS-PRENYLTRANSFERASES; FUNCTIONAL-ANALYSIS; ANALYSIS REVEALS; NATURAL-RUBBER; IN-VITRO; PROTEIN; IDENTIFICATION; ARABIDOPSIS; LATICIFERS;
D O I：
10.1007/s11105-009-0145-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Rubber biosynthesis requires the action of specific enzymes known as cis-prenyltransferases (CPTs). These enzymes are responsible for the sequential addition of isopentenyl pyrophosphate units to the growing polyisoprene chain, a biochemical reaction thought to be stimulated by the presence of small rubber particle proteins (SRPPs). We have cloned, characterized, and analyzed the expression of three CPT genes (TkCPT1-3) and five SRPP genes (TkSRPP1-5) from the rubber-producing plant Taraxacum koksaghyz. The deduced TkCPT amino acid sequences showed significant levels of sequence identity with Hevea brasiliensis CPTs. We also found no obvious differences between SRPPs from T. koksaghyz, another rubber producer, and a non-rubber plant. The roles of the individual TkCPTs and TkSRRPs in rubber biosynthesis are discussed.
引用
收藏
页码：277 / 284
页数：8
相关论文
共 50 条
  • [21] Molecular Cloning and Characterization of Three Genes Encoding Dihydroflavonol-4-Reductase from Ginkgo biloba in Anthocyanin Biosynthetic Pathway
    Cheng Hua
    Li Linling
    Cheng Shuiyuan
    Cao Fuliang
    Xu Feng
    Yuan Honghui
    Wu Conghua
    PLOS ONE, 2013, 8 (08):
  • [22] Uncovering mechanisms of rubber biosynthesis in Taraxacum koksaghyz - role of cis-prenyltransferase-like 1 protein
    Niephaus, Eva
    Mueller, Boje
    van Deenen, Nicole
    Lassowskat, Ines
    Bonin, Martin
    Finkemeier, Iris
    Pruefer, Dirk
    Gronover, Christian Schulze
    PLANT JOURNAL, 2019, 100 (03): : 591 - 609
  • [23] Comparative Transcriptome Analysis in Taraxacum koksaghyz to Identify Genes that Determine Root Volume and Root Length
    Wieghaus, Annika
    Roelfs, Kai-Uwe
    Twyman, Richard M.
    Pruefer, Dirk
    Schulze Gronover, Christian
    FRONTIERS IN GENETICS, 2022, 12
  • [24] Oxidosqualene Cyclase Knock-Down in Latex of Taraxacum koksaghyz Reduces Triterpenes in Roots and Separated Natural Rubber
    van Deenen, Nicole
    Unland, Kristina
    Pruefer, Dirk
    Gronover, Christian Schulze
    MOLECULES, 2019, 24 (15):
  • [25] Molecular cloning and biochemical characterization of a novel cystatin from Hevea rubber latex
    Bangrak, Phuwadol
    Chotigeat, Wilaiwan
    PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2011, 49 (03) : 244 - 250
  • [26] Available exsitu germplasm of the potential rubber crop Taraxacum koksaghyz belongs to a poor rubber producer, T. brevicorniculatum (Compositae–Crepidinae)
    J. Kirschner
    Jan Štěpánek
    Tomáš Černý
    Peter De Heer
    Peter J. van Dijk
    Genetic Resources and Crop Evolution, 2013, 60 : 455 - 471
  • [27] Molecular cloning and characterization of rosmarinic acid biosynthetic genes and rosmarinic acid accumulation in Ocimum basilicum L.
    Kwon, Do Yeon
    Li, Xiaohua
    Kim, Jae Kwang
    Park, Sang Un
    SAUDI JOURNAL OF BIOLOGICAL SCIENCES, 2019, 26 (03) : 469 - 472
  • [28] Molecular cloning and characterization of bitter specific genes from Lupinus angustifolius
    Ikeura, Emi
    Okada, Taketo
    Saito, Kazuki
    Yamazaki, Mami
    PLANT AND CELL PHYSIOLOGY, 2007, 48 : S64 - S64
  • [29] Molecular cloning and characterization of porcine sirtuin genes
    Jin, D.
    Tan, H. J.
    Lei, T.
    Gan, L.
    Chen, X. D.
    Long, Q. Q.
    Feng, B.
    Yang, Z. Q.
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2009, 153 (04): : 348 - 358
  • [30] Available ex situ germplasm of the potential rubber crop Taraxacum koksaghyz belongs to a poor rubber producer, T-brevicorniculatum (Compositae-Crepidinae)
    Kirschner, J.
    Stepanek, Jan
    Cerny, Tomas
    De Heer, Peter
    van Dijk, Peter J.
    GENETIC RESOURCES AND CROP EVOLUTION, 2013, 60 (02) : 455 - 471
12345