IN VITRO EXAMINATION OF THE THROMBOLYTIC EFFICACY OF DESMOTEPLASE AND THERAPEUTIC ULTRASOUND COMPARED WITH RT-PA

被引:8
|
作者
Roessler, Florian C. [1 ]
Wang, Zhihua [2 ,3 ]
Schumacher, Sabrina [4 ]
Ohlrich, Marcus [4 ]
Kaps, Manfred [1 ]
Menciassi, Arianna [2 ,3 ]
Eggers, Juergen [5 ]
机构
[1] Univ Hosp Giessen, Dept Neurol, Giessen, Germany
[2] Scuola Super Sant Anna, BioRobot Inst, Pontedera, Italy
[3] Ist Italiano Tecnol, Ctr Micro BioRobot SSSA, Pontedera, Italy
[4] Univ Hosp Schleswig Holstein, Dept Neurol, Lubeck, Germany
[5] Sana Kliniken Lubeck GmbH, Dept Neurol, Lubeck, Germany
来源
ULTRASOUND IN MEDICINE AND BIOLOGY | 2015年 / 41卷 / 12期
关键词
Cerebrovascular stroke; Sonothrombolysis; Fibrin-selective plasminogen activator; Desmoteplase; Alteplase; Ultrasound; In vitro model; ACUTE ISCHEMIC-STROKE; TISSUE-PLASMINOGEN ACTIVATOR; VAMPIRE BAT; MYOCARDIAL-INFARCTION; LOW-FREQUENCY; BLOOD-CLOTS; SONOTHROMBOLYSIS; RECANALIZATION; FIBRIN; ALTEPLASE;
D O I
10.1016/j.ultrasmedbio.2015.07.007
中图分类号
O42 [声学];
学科分类号
070206 ; 082403 ;
摘要
The aim of the study described here was to evaluate the thrombolytic efficacy of combined treatment with the fibrin-selective plasminogen activator desmoteplase (DSPA) and therapeutic ultrasound (sonothrombolysis [STL]) compared with conventional rt-PA (recombinant tissue plasminogen activator) treatment in vitro. Lysis rates were determined by the weight loss of platelet-rich plasma (PRP) clots treated with rt-PA (60 kU/mL) or DSPA (2 mg/mL) combined with pulsed wave ultrasound (2 MHz, 0.179 W/cm(2)). To reveal the individual effects of medication and ultrasound, lysis rates were also determined for DSPA monotherapy and for combined treatment with rt-PA and ultrasound. Clots solely placed in plasma served as the control group. Lysis increased significantly with rt-PA (26.5 +/- 7.8%) and DSPA (30.5 +/- 6%) compared with the control group (18.2 +/- 5.9%) (each p, 0.001). DSPA lysis was more effective than rt-PA lysis (without STL: p = 0.015, with STL: p = 0.01). Combined treatment with DSPA and 2-MHz STL significantly exceeded rt-PA lysis (32.8% vs. 26.5%, p < 0.001). (C) 2015 World Federation for Ultrasound in Medicine & Biology.
引用
收藏
页码:3233 / 3240
页数:8
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