Molecular cloning and phylogenetic analysis of canine β-casein

被引：2
|
作者：
Watanabe, M
Sugano, S
Togashi, T
Imai, JI
Uchida, K
Yamaguchi, R
Tateyama, S
机构：
[1] Univ Tokyo, Inst Med Sci, Dept Virol, Minato Ku, Tokyo 1088639, Japan
[2] Miyazaki Univ, Fac Agr, Dept Vet Pathol, Miyazaki 8892155, Japan
来源：
DNA SEQUENCE | 2000年 / 11卷 / 3-4期
关键词：
beta-casein; degenerate oligo primer; dog; RT-PCR;
D O I：
10.3109/10425170009033246
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A canine beta -casein cDNA was isolated from mammary tissue by polymerase chain reaction (PCR) using degenerate primers. It encodes 250 amino acids protein containing the conserved sequence motif of beta -casein. It showed the highest homology with snow-leopard (Uncia uncia) (55-62 % identity). It also showed 44-53% identity with human, 33-42 % identity with mouse, 29-37% identity with rat, 43-53 % identity with rabbit, 41-48% identity with pig, 44-51% identity with cattle and 44-50 % identity with sheep. A 1.2-kb mRNA was detected in mammary tissue by Northern blot analysis. Phylogenetic analysis revealed that canine beta -casein formed a branch with lesser panda and snow leopard, which were grouped into carnivore.
引用
收藏
页码：295 / 300
页数：6
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