Two-photon excitation fluorescence microscopy

被引：883
|
作者：
So, PTC [1 ]
Dong, CY
Masters, BR
Berland, KM
机构：
[1] MIT, Dept Mech Engn, Cambridge, MA 02139 USA
[2] Emory Univ, Dept Phys, Atlanta, GA 30322 USA
[3] Univ Bern, Dept Ophthalmol, Bern, Switzerland
来源：
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING | 2000年 / 2卷
关键词：
multiphoton; fluorescence spectroscopy; single molecule; functional imaging; tissue imaging;
D O I：
10.1146/annurev.bioeng.2.1.399
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Two-photon fluorescence microscopy is one of the most important recent inventions in biological imaging. This technology enables noninvasive study of biological specimens in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons. This excitation process has a number of unique advantages, such as reduced specimen photodamage and enhanced penetration depth. It also produces higher-contrast images and is a novel method to trigger localized photochemical reactions. Two-photon microscopy continues to find an increasing number of applications in biology and medicine.
引用
收藏
页码：399 / 429
页数：39
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