Actinotalea caeni sp nov., isolated from a sludge sample of a biofilm reactor

A polyphasic taxonomic study was carried out on strain EBR-4-2(T) isolated from a biofilm reactor in Korea. Cells of the strain were Gram-stain-positive, non-spore-forming, non-motile and rod-shaped. Comparative 16S rRNA gene sequence studies showed the clear affiliation of this strain to the Actinobacteria, and it had the highest pairwise sequence similarities with Actinotalea suaedae EGI 60002(T) (98.7 %), Actinotalea ferrariae CF5-4(T) (96.3 %) and Actinotalea fermentans DSM 3133(T) (96.2 %). Phylogenetic analysis based on the 16S rRNA gene sequences showed that the strain formed a clear phylogenetic lineage with the genus Actinotalea. The major fatty acids were identified as C-15 : 0 anteiso, C-16 : 0, C1(6 : 0) N alcohol, C-15 : 1 anteiso A and C-15 : 0 iso. The major cellular polar lipids were diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol and glycolipid. The peptidoglycan type was A4b containing L-Orn-D-Glu. The whole-cell-wall sugars were glucose and ribose. The respiratory quinone was identified as menaquinone MK-10(H-4), and the genomic DNA G+C content was determined to be 74.8 mol %. Based on evidence from this polyphasic study, it is proposed that strain EBR-4-2(T) should be designated as representing a novel species named Actinotalea caeni sp. nov. The type stain is EBR-4-2(T) (=KCTC 33604 T=JCM 30447(T)).