It is well known that alkali scouring is ecologically and economically unfavorable; therefore in last Jew years new procedures of enzymatic scouring with pectinases were investigated. The scouring with alkali pectinases results in better sewability. Recently, the new neutral pectinases were introduced. In this paper, the influence of these pectinase scouring the cotton knitted fabric sewability was researched, and compared to traditional alkali, and alkali pectinase scouring, as well. Therefore, raw cotton knitted fabric was scoured in three different ways - traditional with alkali, and enzymatic with two pectinases - - BioPrep 3000L (Novozymes), alkali one and Beisol PRO (Bezema). neutral (me; bleached and softened. The influence of these treatments to wastewater quality, and to cotton fabric properties such are hydrophilicity, degree of polymerization (DP), whiteness and needle penetration farce are compared. Damage of knitted fabric loops during sewing procedure through two and four layers, which are closely connected with the values of sewing needle penetration force and dynamic tension of the sewing thread, were measured on new developed instrument which detects,faults in samples of the knitted fabric by means of sensors.