Isolation and chromosomal mapping of the human homolog of perilipin (PLIN), a rat adipose tissue-specific gene, by differential display method

被引：32
|
作者：
Nishiu, J [1 ]
Tanaka, T [1 ]
Nakamura, Y [1 ]
机构：
[1] Univ Tokyo, Inst Med Sci, Ctr Human Genome, Mol Med Lab,Minato Ku, Tokyo 108, Japan
来源：
GENOMICS | 1998年 / 48卷 / 02期
基金：
日本学术振兴会;
关键词：
D O I：
10.1006/geno.1997.5179
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Using the differential display technique, we isolated a cDNA clone encoding the human homolog of rat perilipin, a unique protein associated with intracellular neutral lipid droplets in adipocytes and steroidogenic cells. The full cDNA contains an open reading frame of 1566 nucleotides encoding 522 amino acids and bears 79% amino acid identity to rat perilipin, Northern blot analysis showed that among 20 human adult tissues examined, human perilipin was expressed specifically in adipose tissues, We determined the chromosomal location of the perilipin gene at 15q26 by means of fluorescence in situ hybridization. (C) 1998 Academic Press.
引用
收藏
页码：254 / 257
页数：4
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