Alternative sources of energy are greatly needed to ensure the availability of fuels in the long run. Microalgae-derived fuels are among the viable options due to their potential to produce sustainable fuels. However, the costs related to the production of microalgal and alternative fuels are still higher compared to conventional fuels. To deal with costs and environmental issues, the blending of microalgal fuels with conventional fuels can be considered as one of the feasible options. Blending has a positive impact on many fuel characteristics including reduction of carbon footprint, costs, freezing point, viscosity, flow, heating and combustion. In this study, jet fuel from Nannochloropsis sp crude bio-oil was blended with Jet A1 in 50/50 volume ratio. The data generated from the 50/50 blend jet fuel were analysed according to the ASTM standards. Samples were tested after 30, 60, 90 and 120 days from the production day. It was reported that majority of parameters including Net heat of combustion, flash point, kinematic viscosity, conductivity and freezing point were compliant with ASTM standards. However, parameters such as density and total acidity were found not complying with ASTM standards. This study can have an implication on carbon footprint reduction because of the blending of fossil-based jet fuel and renewable-based jet fuel known as low carbon footprint fuel. The blending ratio can be beneficial for the green energy world in terms of costs and environmental impacts.