A Rat Monoclonal Antibody Against the Chromatin Remodeling Factor CHD5

被引：6
|
作者：
Yoshimura, Saori
Yoshimi, Tomohiko
Ohkawa, Yasuyuki [2 ]
Azuma, Masayuki
Tachibana, Taro [1 ]
机构：
[1] Osaka City Univ, Dept Bioengn, Grad Sch Engn, Sumiyoshi Ku, Osaka 5588585, Japan
[2] Kyushu Univ, Fac Med, Dept Epigenet, SSP Stem Cell Unit, Fukuoka 812, Japan
来源：
HYBRIDOMA | 2010年 / 29卷 / 01期
关键词：
DIVERSE; CANCER; FAMILY;
D O I：
10.1089/hyb.2009.0069
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
CHD5 (chromodomain/helicase/DNA-binding protein 5) is a member of the CHD subfamily of chromatin remodeling Swi/Snf proteins, and has been recently identified as a tumor suppressor in a diverse range of human cancers. We report here on the establishment of a hybridoma cell line for producing a monoclonal antibody against CHD5 by the rat medial iliac lymph node method. Immunoblotting analyses indicated that this antibody, MAb 5A10, specifically recognizes endogenous CHD5. In immunostaining using the antibody, a nuclear staining pattern was observed. The monoclonal antibody will be useful in immunoblotting and immunolocalization experiments in a variety of cells and tissues, as well as in further studies of the biological function and cellular dynamics of this protein.
引用
收藏
页码：63 / 66
页数：4
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