Complete NMR assignment of a bisecting hybrid-type oligosaccharide transferred by Mucor hiemalis endo-β-N-acetylglucosaminidase

被引：2
|
作者：
Yamanoi, Takashi [1 ]
Oda, Yoshiki [2 ]
Katsuraya, Kaname [3 ]
Inazu, Toshiyuki [4 ]
Yamamoto, Kenji [5 ]
机构：
[1] Josai Univ, Fac Pharmaceut Sci, 1-1 Keyakidai, Sakado, Saitama 3500295, Japan
[2] Tokai Univ, Technol Joint Management Off, 4-1-1 Kitanakame, Hiratsuka, Kanagawa 2591292, Japan
[3] Wayo Womens Univ, Dept Human Ecol, Chiba 2728533, Japan
[4] Tokai Univ, Inst Glycosci, Sch Engn, Dept Appl Chem, 4-1-1 Kitanakame, Hiratsuka, Kanagawa 2591292, Japan
[5] Ishikawa Prefectural Univ, Res Inst Bioresources & Biotechnol, Nonoichi, Ishikawa 9218836, Japan
来源：
CARBOHYDRATE RESEARCH | 2016年 / 427卷
关键词：
NMR spectral assignment; Bisecting hybrid-type oligosaccharide; Endo-beta-N-acetylglucosaminidase; Endo-M; Transglycosylation product; CHEMOENZYMATIC SYNTHESIS;
D O I：
10.1016/j.carres.2016.03.013
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This study describes the complete nuclear magnetic resonance (NMR) spectral assignment of a bisecting hybrid-type oligosaccharide 1, transferred by Mucor hiemalis endo-beta-N-acetylglucosaminidase (Endo-M). Through H-1- and C-13-NMR, DQF-COSY, HSQC, HMBC, TOCSY, and NOESY experiments, we determine the structure of the glycoside linkage formed by the Endo-M transglycosylation, i.e., the connection between GlcNAc and GlcNAc in oligosaccharide 1. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：60 / 65
页数：6
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