Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy

被引：6

作者：

Suhling, Klaus ^{[1
]}

Hirvonen, Liisa M. ^{[1
]}

Becker, Wolfgang ^{[2
]}

Smietana, Stefan ^{[2
]}

Netz, Holger ^{[2
]}

Milnes, James ^{[3
]}

Conneely, Thomas ^{[3
]}

Le Marois, Alix ^{[1
]}

Jagutzki, Ottmar ^{[4
]}

机构：

[1] Kings Coll London, Dept Phys, London WC2R 2LS, England

[2] Becker & Hickl GmbH, Nahmitzer Damm 30, D-12277 Berlin, Germany

[3] Photek Ltd, 26 Castleham Rd, St Leonards On Sea TN38 9NS, England

[4] Inst Kernphys, Max von Laue Str 1, D-60438 Frankfurt, Germany

来源：

ADVANCED PHOTON COUNTING TECHNIQUES X | 2016年 / 9858卷

关键词：

Time-correlated single photon counting (TCSPC); fluorescence lifetime imaging (FLIM); phosphorescence lifetime imaging (PLIM); single photon detection; microchannel plate (MCP); delay line anode; single photon avalanche photodiode (SPAD); SPAD array; MICROCHANNEL-PLATE DETECTORS; MICROSECOND TIME RESOLUTION; DNA MICROARRAYS; EXPOSURE TIME; PHOTON; CAMERA; SPECTROSCOPY; DOMAIN; SENSOR; SPEED;

D O I：

10.1117/12.2227198

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Time-correlated single photon counting (TCSPC) is a widely used, sensitive, precise, robust and mature technique to measure photon arrival times in applications such as fluorescence spectroscopy and microscopy, light detection and ranging (lidar) and optical tomography. Wide-field TCSPC detection techniques, where the position and the arrival time of the photons are recorded simultaneously, have seen several advances in the last few years, from the microsecond to the picosecond time scale. Here, we summarise some of our recent work in this field with emphasis on microsecond resolution phosphorescence lifetime imaging (PLIM) and nanosecond fluorescence lifetime imaging (FLIM) microscopy.

引用

页数：10

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