Detection of hilA gene sequences in serovars of Salmonella enterica subspecies enterica

被引：45

作者：

Cardona-Castro, N

Restrepo-Pineda, E

Correa-Ochoa, M

机构：

[1] Inst Colombiano Med Trop, Microbiol Lab, Sabaneta 52153, Antioquia, Colombia

[2] Univ Antioquia, Escuela Bacteriol, Medellin, Colombia

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2002年 / 97卷 / 08期

关键词：

hilA gene sequences; Salmonella serovars Typhi; Enteritidis; Choleraesuis; Paratyphi A and B; Pullorum;

D O I：

10.1590/S0074-02762002000800016

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

hilA gene promoter component of the Salmonella Pathogenicity Island 1, has been found in Salmonella serovar Typhimurium, being important for the regulation of type III secretion apparatus genes. We detected hilA gene sequences in Salmonella serovars Typhi, Enteritidis, Choleraesuis, Paratyphi A and B, and Pullorum, by polymerase chain reaction (PCR) and hybridization techniques. The primers to carry out PCR were designed according to hilA sequence. A low stringency hybridization with the probe pVV441 (hilA open-reading-frame plasmid) was carried out. To find hilA gene sequences in other Salmonella sp. suggest that these serovars could have similar sequences of this kind of virulence genes.

引用

页码：1153 / 1156

页数：4

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