New Horizons in Reversed-Phase Chromatography Selectivity

For decades, reversed-phase (RP) chromatography has dominated HPLC applications. Almost as soon as bonded reversed phases became available in the early 1970s, this mode surged ahead as the most popular. Through the years, the technique has become more efficient, columns more stable, and new types of phases beyond the traditional C8 and C18 give different selectivity that is required for more difficult separations. Here, we focus on the characterization of reversed-phase columns with particular emphasis on the hydrophobic-subtraction model. A well-attended Pittcon 2010 session brought together many experts to update our knowledge in this area and help liquid chromatographers make more educated decisions on phase selection and usage.