Differential Proteomics Identification of HSP90 as Potential Serum Biomarker in Hepatocellular Carcinoma by Two-dimensional Electrophoresis and Mass Spectrometry

被引：43
|
作者：
Sun, Yiyi [2 ]
Zang, Zhihe [2 ]
Xu, Xiaohong [2 ]
Zhang, Zhonglin [2 ]
Zhong, Ling [2 ]
Zan, Wang [2 ]
Zhao, Yan [2 ]
Sun, Lin [1 ]
机构：
[1] Sichuan Univ, W China Hosp, Chengdu 610041, Sichuan Prov, Peoples R China
[2] Chengdu Med Coll, Chengdu 610083, Sichuan Prov, Peoples R China
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2010年 / 11卷 / 04期
关键词：
proteomics; hepatocellular carcinoma; serum biomarker; IMMOBILIZED PH GRADIENTS; SHOCK-PROTEIN; 90; GEL-ELECTROPHORESIS; COLORECTAL-CANCER; GENE-EXPRESSION; EARLY-DIAGNOSIS; BREAST-CANCER; TUMOR-MARKERS; EPIDEMIOLOGY; INHIBITORS;
D O I：
10.3390/ijms11041423
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The aim of the current study is to identify the potential biomarkers involved in Hepatocellular carcinoma (HCC) carcinogenesis. A comparative proteomics approach was utilized to identify the differentially expressed proteins in the serum of 10 HCC patients and 10 controls. A total of 12 significantly altered proteins were identified by mass spectrometry. Of the 12 proteins identified, HSP90 was one of the most significantly altered proteins and its over-expression in the serum of 20 HCC patients was confirmed using ELISA analysis. The observations suggest that HSP90 might be a potential biomarker for early diagnosis, prognosis, and monitoring in the therapy of HCC. This work demonstrates that a comprehensive strategy of proteomic identification combined with further validation should be adopted in the field of cancer biomarker discovery.
引用
收藏
页码：1423 / 1433
页数：11
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