共 31 条
Long-term survival of the mouse ES cell-derived mast cell, MEDMC-BRC6, in mast cell-deficient Kitw-sh/w-sh mice
被引：1
|作者：
Shibagaki, Shohei
[1
,2
]
Tahara-Hanaoka, Satoko
[1
,3
]
Hiroyama, Takashi
[4
]
Nakamura, Yukio
[4
]
Shibuya, Akira
[1
,3
]
机构：
[1] Univ Tsukuba, Fac Med, Dept Immunol, 1-1-1 Ten Nodai, Tsukuba, Ibaraki 3058575, Japan
[2] Univ Tsukuba, Grad Sch Comprehens Human Sci, Doctoral Program, 1-1-1 Ten Nodai, Tsukuba, Ibaraki 3058575, Japan
[3] Univ Tsukuba, Life Sci Ctr, TARA, 1-1-1 Ten Nodai, Tsukuba, Ibaraki 3058575, Japan
[4] RIKEN, BioResource Ctr, Cell Engn Div, 3-1-1 Koyadai, Tsukuba, Ibaraki 3050074, Japan
基金：
日本学术振兴会;
关键词：
degranulation;
Fc epsilon RI;
IgE-dependent anaphylaxis;
TLR2;
TLR4;
EMBRYONIC STEM-CELLS;
IN-VIVO;
KIT;
ACTIVATION;
ALLERGIN-1;
RECEPTOR;
CULTURE;
UNIQUE;
MODEL;
D O I：
10.1093/intimm/dxx022
中图分类号：
R392 [医学免疫学];
Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Mast cells (MCs) play pivotal roles in allergic reactions and the host defense against microbial infection through the IgE-dependent and IgE-independent signaling pathways. MC lines that can be analyzed both in vitro and in vivo would be useful for the study of MC-dependent immune responses. Here, we investigated the functional characteristics of a mouse embryonic stem cell-derived MC-like cell line, MEDMC-BRC6. The cell line expressed Fc epsilon RI and c-Kit and showed degranulation and production of inflammatory cytokines and chemokines, including TNF-alpha, IL-6 and MCP-1, upon cross-linking FceRI with IgE. These cytokines and chemokines were also produced by the cell line by stimulation of TLR2 and TLR4. MEDMC-BRC6 survived in the peritoneal cavity and the ear skin for at least 6 months after the transfer into genetically compatible MC-deficient Kit(W-sh/W-sh) mice, in which systemic anaphylaxis was successfully induced. Thus, MEDMC-BRC6 cells represent a potent tool for investigating the functions of MCs in vitro and in vivo.
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页码：235 / 242
页数：8
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