Enzyme powder amylase complex from Aspergillus awamori KT-11 for hydrolysis of cassava (Manihot esculenta)

Glucoamylase is the common enzyme to produce liquid sugar from starch. This enzyme breaks the starch chain randomly. Enzymes that are commonly used are liquid enzymes. Liquid enzymes stored at room temperature are easily damaged. The aim of the present study is to check the ability of the glucoamylase powder enzyme from Aspergillus awamori KT-11 in hydrolyzing starch-based biomass for producing liquid sugar. The produced liquid sugar was analyzed its concentration of reducing sugar, glucose concentration, sweetness level, and micrograph as a result of hydrolysis of biomass by enzyme glucoamylase. Variations concentration of the glucoamylase enzyme with cassava juice substrate was optimized to produce a high concentration of liquid sugar. The optimal time on hydrolysing 30% cassava with the enzyme is at 48 hours with enzyme concentration of 10.0469 U and substrate concentration of 30%. The sweetness level obtained on the refractometer is 4.2 % Brix. Approximately, 4000 ppm of glucose can be produced after 48 hours of reaction.